mouse anti pp38  (Cell Signaling Technology Inc)

Journal: Applied Biological Chemistry

Article Title: Assessing the therapeutic potential of Ganoderma lucidum spore oil in alleviating periodontal tissue damage in murine periodontitis model

doi: 10.1186/s13765-024-00941-3

Figure Lengend Snippet: Fig. 3 GLSO regulates the expression of inflammatory factors by suppressing the JNK pathway activated by IL-1β in hGFs. A Western blot results of ERK, p38, JNK, and their phosphorylated forms (pERK, pp38, pJNK) and IκBα in hGFs treated with 2 ng/ml IL-1β for the indicated time (n ≥ 3). B Protein levels of pERK, pJNK and IκBα in hGFs treated with GLSO in presence of the 2 ng/ml IL-1β for 15 min (n ≥ 3). C qRT-PCR analysis of MMP1 and IL8 mRNA expression levels in hGFs treated with SP600125 (JNK inhibitor) in the presence of 2 ng/ml IL-1β for 24 h (n = 9). Data are presented as mean ± SD. n indicates the number of biologically independent experiments. Statistical analyses were performed using Kruskal–Wallis test (MMP-1 of C) and one-way ANOVA followed by Games-Howell’s post-hoc test (IL-8 of C). *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: The membrane was blocked with 5% skim milk for 1 h and subsequently incubated with the indicated primary antibodies for overnight, including anti-MMP-1 (Novus, NBP2-22123), anti-IL-8 (Cell signaling, #94407S), anti-pERK (Cell Signaling, #9101), anti-ERK (BD Biosciences, 610408), anti-pp38 (Cell Signaling, #9216), anti-p38 (Santa Cruz, sc-535), anti-pJNK (Cell Signaling, #9255), anti-JNK (Cell Signaling, #9252), anti-IκBα (Santa Cruz, sc-371), and antiβ-ACTIN (Sigma-Aldrich, A3854) as a loading control.

Techniques: Expressing, Western Blot, Quantitative RT-PCR